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sort == 'palette' % % for worth in aspect.values % % endfor % % elsif facet.type == 'slider' % % if aspect.field contains 'rate' % % else % % endif %

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Molecular basis for the enzymatic inactivity of class III glutaredoxin ROXY9 on regular glutathionylated substrates

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, Pretty much no info is accessible for course III GRXs. This has actually been due to encountered troubles when purifying recombinant proteins expressed in E. coli30. Below, we succeeded in getting milligram amounts of class III GRX ROXY9 from Arabidopsis thaliana by making use of the baculovirus expression technique in insect cells.

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The amino acid environments of those residues as located in sequences representing all three GRX lessons encoded from the Arabidopsis genome are proven in Fig. 1b. The alignment highlights that course III GRXs will not encode the class II-specific 5 amino acid loop which interferes with oxidoreductase activity14,15, nor the roxy 9 proline in the Lively web site which might interfere with FeS cluster assembly16.

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